Interleukin-1β induction in the human monocytic cell line THP-1 by lipooligosaccharides of campylobacter species

Activity: Academic Talks or PresentationsOral presentationResearch

Description

The Campylobacter lipooligosaccharides (LOS) can stimulate membrane bound innate immune receptors in human macrophages. However, the association of Campylobacter LOS in the stimulation of cytosolic receptors or the inflammasome remains poorly characterised. Therefore, we determined the role of Campylobacter jejuni and Campylobacter coli LOS in the activation of NLRP3 inflammasome-dependent signalling in human macrophages. Induction of NLRP3 inflammasomemediated interleukin-1β (IL-1β) and Caspase-1 secretion in THP-1 supernatants was quantified using ELISA following co-culture of THP-1 cells with LOS extracts from wild-type C. jejuni 11168, mutant C. jejuni 11168, C. coli RM1875 and C. coli 76339. Our results demonstrated that both C. jejuni and C. coli purified LOS can induce Caspase-1 and IL-1β production in THP-1 cells. However, C. jejuni 11168 mutant LOS with modified lipid A and a lack of core oligosaccharides stimulated significantly reduced Caspase-1 and IL-1β. This result was also replicated in co-culture of live wild-type and mutant C. jejuni with THP-1 cells. This study provides new insights into the interaction of Campylobacter with human macrophages and suggests that NLRP3 inflammasome activation in human macrophages can alter because of variation in Campylobacter LOS structure
Period20 Jun 201924 Jun 2019
Event titleAmerican Society for Microbiology : Microbe 2019
Event typeConference
LocationSan Francisco, United States
Degree of RecognitionInternational