Abstract
The effect of oestrogen (17β –oestradiol) on cancer cell proliferation in mice and humans has been evaluated in numerous studies. However, the effect of this steroid hormone on melanoma cells remains controversial. Physiological and
pathological processes related linked to reproduction, skin, and the immune system may be affected by 17β- oestradiol. Therefore, employing the commonly used murine melanoma cells B16-F10, we investigated the effects of 17β- oestradiol on cell activity, viability and determined expression of oestrogen receptors (ERs) α and β . At non-cytotoxic concentrations of oestrogen there were significant increases in mitochondrial cell activity and viability in a dose dependent manner at 48- and 72-hours post-treatment. Furthermore, we demonstrated that B16-F10 cells express oestrogen receptor α and β mRNA. Quantitative PCR results showed significant up-regulation of ER β expression and concomitant down regulation of ER α expression in cells treated with 17β- oestradiol cells. These findings deepen our mechanistic understanding of the impact of oestrogen (17β- oestradiol) on the activity and proliferation of the commonly employed melanoma cell line B16-F10. This work highlights the potential role of oestrogen signalling in melanoma progression, paving the way for targeted therapeutic strategies in melanoma treatment.
pathological processes related linked to reproduction, skin, and the immune system may be affected by 17β- oestradiol. Therefore, employing the commonly used murine melanoma cells B16-F10, we investigated the effects of 17β- oestradiol on cell activity, viability and determined expression of oestrogen receptors (ERs) α and β . At non-cytotoxic concentrations of oestrogen there were significant increases in mitochondrial cell activity and viability in a dose dependent manner at 48- and 72-hours post-treatment. Furthermore, we demonstrated that B16-F10 cells express oestrogen receptor α and β mRNA. Quantitative PCR results showed significant up-regulation of ER β expression and concomitant down regulation of ER α expression in cells treated with 17β- oestradiol cells. These findings deepen our mechanistic understanding of the impact of oestrogen (17β- oestradiol) on the activity and proliferation of the commonly employed melanoma cell line B16-F10. This work highlights the potential role of oestrogen signalling in melanoma progression, paving the way for targeted therapeutic strategies in melanoma treatment.
| Original language | English |
|---|---|
| Journal | Journal of Bioscience and Applied Research |
| Volume | 11 |
| Publication status | Accepted/In press - 7 May 2025 |
Data Access Statement
All data generated or analysed in this study are included in this published article and its supplementary materials. No additional datasets were used or created for this study.Keywords
- 17β- oestradiol
- oestrogen receptors
- B16-F10
- Melanoma
