ALG-2 interacting protein AIP1: A novel link between D1 and D3 signalling

Lingping Zhan, Bigang Liu, Maria Jose-Lafuente, Margarita V. Chibalina, Andrew Grierson, Alan Maclean, Jamal Nasir

    Research output: Contribution to journalArticle

    Abstract

    Dopamine signalling is a critically important process in the human brain that controls mood, cognition and motor activity. In order to gain detailed insight into this signalling pathway at the molecular level, we carried out yeast two-hybrid screens with D1-like (D1, D5) and D2-like (D2, D3, D4) dopamine receptors and identified 11 dopamine receptor interacting proteins (DRIPs). Using the C-terminal domain of D1 receptor as bait, we identified AIP1 (ALG-2 interacting protein 1), a known modulator of caspase-dependent and caspase-independent cell death, including neuronal cell death, that is also part of the endosomal transport system. In a separate yeast two-hybrid screen, using the third intracellular cytoplasmic loop of D3 as bait, we again identified AIP1. The interaction of AIP1 with both D1 and D3 was confirmed in vitro and in vivo using a variety of methods, including glutathione S-transferase (GST) pull-down, blot overlay and coimmunoprecipitation from mouse brain lysates. We have also observed colocalization of D1 and D3 with AIP1 in mouse brain tissue. In addition, coexpression of AIP1 with D1 resulted in > 50% reduction in binding capacity of D1 to its antagonist. Finally, AIP1 up-regulates D1 and D3 expression and appears to be important for their stability and trafficking.
    Original languageEnglish
    Pages (from-to)1626-1633
    Number of pages8
    JournalEuropean Journal of Neuroscience
    Volume27
    Issue number7
    DOIs
    Publication statusPublished - Apr 2008

    Fingerprint

    Caspases
    Proteins
    Brain
    Cell Death
    Yeasts
    Dopamine D4 Receptors
    Receptor-Interacting Protein Serine-Threonine Kinases
    Dopamine D3 Receptors
    Dopamine Receptors
    Glutathione Transferase
    Cognition
    Dopamine
    Motor Activity
    Up-Regulation
    In Vitro Techniques
    Mouse Pdcd6ip protein

    Keywords

    • Dopamine receptors
    • Homo sapiens
    • Psychiatric genetics
    • Schizophrenia
    • Yeast two-hybrid

    Cite this

    Zhan, L., Liu, B., Jose-Lafuente, M., Chibalina, M. V., Grierson, A., Maclean, A., & Nasir, J. (2008). ALG-2 interacting protein AIP1: A novel link between D1 and D3 signalling. European Journal of Neuroscience, 27(7), 1626-1633. https://doi.org/10.1111/j.1460-9568.2008.06135.x
    Zhan, Lingping ; Liu, Bigang ; Jose-Lafuente, Maria ; Chibalina, Margarita V. ; Grierson, Andrew ; Maclean, Alan ; Nasir, Jamal. / ALG-2 interacting protein AIP1: A novel link between D1 and D3 signalling. In: European Journal of Neuroscience. 2008 ; Vol. 27, No. 7. pp. 1626-1633.
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    Zhan, L, Liu, B, Jose-Lafuente, M, Chibalina, MV, Grierson, A, Maclean, A & Nasir, J 2008, 'ALG-2 interacting protein AIP1: A novel link between D1 and D3 signalling', European Journal of Neuroscience, vol. 27, no. 7, pp. 1626-1633. https://doi.org/10.1111/j.1460-9568.2008.06135.x

    ALG-2 interacting protein AIP1: A novel link between D1 and D3 signalling. / Zhan, Lingping; Liu, Bigang; Jose-Lafuente, Maria; Chibalina, Margarita V.; Grierson, Andrew; Maclean, Alan; Nasir, Jamal.

    In: European Journal of Neuroscience, Vol. 27, No. 7, 04.2008, p. 1626-1633.

    Research output: Contribution to journalArticle

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    AU - Zhan, Lingping

    AU - Liu, Bigang

    AU - Jose-Lafuente, Maria

    AU - Chibalina, Margarita V.

    AU - Grierson, Andrew

    AU - Maclean, Alan

    AU - Nasir, Jamal

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    KW - Homo sapiens

    KW - Psychiatric genetics

    KW - Schizophrenia

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    Zhan L, Liu B, Jose-Lafuente M, Chibalina MV, Grierson A, Maclean A et al. ALG-2 interacting protein AIP1: A novel link between D1 and D3 signalling. European Journal of Neuroscience. 2008 Apr;27(7):1626-1633. https://doi.org/10.1111/j.1460-9568.2008.06135.x