Fat mass and obesity associated (FTO) gene influences skeletal muscle phenotypes in non-resistance trained males and elite rugby playing position

S M Heffernan, G K Stebbings, L P Kilduff, R M Erskine, S H Day, C I Morse, J S McPhee, C J Cook, B Vance, William J Ribbans, Stuart M Raleigh, C Roberts, M A Bennett, G Wang, M Collins, Y P Pitsiladis, A G Williams

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background FTO gene variants have been associated with obesity phenotypes in sedentary and obese populations, but rarely with skeletal muscle and elite athlete phenotypes. Methods In 1089 participants, comprising 530 elite rugby athletes and 559 non-athletes, DNA was collected and genotyped for the FTO rs9939609 variant using real-time PCR. In a subgroup of non-resistance trained individuals (NT; n = 120), we also assessed structural and functional skeletal muscle phenotypes using dual energy x-ray absorptiometry, ultrasound and isokinetic dynamometry. In a subgroup of rugby athletes (n = 77), we assessed muscle power during a countermovement jump. Results In NT, TT genotype and T allele carriers had greater total body (4.8% and 4.1%) and total appendicular lean mass (LM; 3.0% and 2.1%) compared to AA genotype, with greater arm LM (0.8%) in T allele carriers and leg LM (2.1%) for TT, compared to AA genotype. Furthermore, the T allele was more common (94%) in selected elite rugby union athletes (back three and centre players) who are most reliant on LM rather than total body mass for success, compared to other rugby athletes (82%; P = 0.01, OR = 3.34) and controls (84%; P = 0.03, OR = 2.88). Accordingly, these athletes had greater peak power relative to body mass than other rugby athletes (14%; P = 2 x 10-6). Conclusion Collectively, these results suggest that the T allele is associated with increased LM and elite athletic success. This has implications for athletic populations, as well as conditions characterised by low LM such as sarcopenia and cachexia.
Original languageEnglish
JournalBMC Genetics
Volume18
Issue number4
DOIs
Publication statusPublished - 19 Jan 2017

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Football
Athletes
Skeletal Muscle
Obesity
Fats
Phenotype
Genes
Alleles
Genotype
Sports
Sarcopenia
Cachexia
Population
Real-Time Polymerase Chain Reaction
Leg
Arm
X-Rays
Muscles
DNA

Keywords

  • IRX3
  • Lean mass
  • RugbyGene project

Cite this

Heffernan, S. M., Stebbings, G. K., Kilduff, L. P., Erskine, R. M., Day, S. H., Morse, C. I., ... Williams, A. G. (2017). Fat mass and obesity associated (FTO) gene influences skeletal muscle phenotypes in non-resistance trained males and elite rugby playing position. BMC Genetics, 18(4). https://doi.org/10.1186/s12863-017-0470-1
Heffernan, S M ; Stebbings, G K ; Kilduff, L P ; Erskine, R M ; Day, S H ; Morse, C I ; McPhee, J S ; Cook, C J ; Vance, B ; Ribbans, William J ; Raleigh, Stuart M ; Roberts, C ; Bennett, M A ; Wang, G ; Collins, M ; Pitsiladis, Y P ; Williams, A G. / Fat mass and obesity associated (FTO) gene influences skeletal muscle phenotypes in non-resistance trained males and elite rugby playing position. In: BMC Genetics. 2017 ; Vol. 18, No. 4.
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abstract = "Background FTO gene variants have been associated with obesity phenotypes in sedentary and obese populations, but rarely with skeletal muscle and elite athlete phenotypes. Methods In 1089 participants, comprising 530 elite rugby athletes and 559 non-athletes, DNA was collected and genotyped for the FTO rs9939609 variant using real-time PCR. In a subgroup of non-resistance trained individuals (NT; n = 120), we also assessed structural and functional skeletal muscle phenotypes using dual energy x-ray absorptiometry, ultrasound and isokinetic dynamometry. In a subgroup of rugby athletes (n = 77), we assessed muscle power during a countermovement jump. Results In NT, TT genotype and T allele carriers had greater total body (4.8{\%} and 4.1{\%}) and total appendicular lean mass (LM; 3.0{\%} and 2.1{\%}) compared to AA genotype, with greater arm LM (0.8{\%}) in T allele carriers and leg LM (2.1{\%}) for TT, compared to AA genotype. Furthermore, the T allele was more common (94{\%}) in selected elite rugby union athletes (back three and centre players) who are most reliant on LM rather than total body mass for success, compared to other rugby athletes (82{\%}; P = 0.01, OR = 3.34) and controls (84{\%}; P = 0.03, OR = 2.88). Accordingly, these athletes had greater peak power relative to body mass than other rugby athletes (14{\%}; P = 2 x 10-6). Conclusion Collectively, these results suggest that the T allele is associated with increased LM and elite athletic success. This has implications for athletic populations, as well as conditions characterised by low LM such as sarcopenia and cachexia.",
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author = "Heffernan, {S M} and Stebbings, {G K} and Kilduff, {L P} and Erskine, {R M} and Day, {S H} and Morse, {C I} and McPhee, {J S} and Cook, {C J} and B Vance and Ribbans, {William J} and Raleigh, {Stuart M} and C Roberts and Bennett, {M A} and G Wang and M Collins and Pitsiladis, {Y P} and Williams, {A G}",
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Heffernan, SM, Stebbings, GK, Kilduff, LP, Erskine, RM, Day, SH, Morse, CI, McPhee, JS, Cook, CJ, Vance, B, Ribbans, WJ, Raleigh, SM, Roberts, C, Bennett, MA, Wang, G, Collins, M, Pitsiladis, YP & Williams, AG 2017, 'Fat mass and obesity associated (FTO) gene influences skeletal muscle phenotypes in non-resistance trained males and elite rugby playing position', BMC Genetics, vol. 18, no. 4. https://doi.org/10.1186/s12863-017-0470-1

Fat mass and obesity associated (FTO) gene influences skeletal muscle phenotypes in non-resistance trained males and elite rugby playing position. / Heffernan, S M; Stebbings, G K; Kilduff, L P; Erskine, R M; Day, S H; Morse, C I; McPhee, J S; Cook, C J; Vance, B; Ribbans, William J; Raleigh, Stuart M; Roberts, C; Bennett, M A; Wang, G; Collins, M; Pitsiladis, Y P; Williams, A G.

In: BMC Genetics, Vol. 18, No. 4, 19.01.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Fat mass and obesity associated (FTO) gene influences skeletal muscle phenotypes in non-resistance trained males and elite rugby playing position

AU - Heffernan, S M

AU - Stebbings, G K

AU - Kilduff, L P

AU - Erskine, R M

AU - Day, S H

AU - Morse, C I

AU - McPhee, J S

AU - Cook, C J

AU - Vance, B

AU - Ribbans, William J

AU - Raleigh, Stuart M

AU - Roberts, C

AU - Bennett, M A

AU - Wang, G

AU - Collins, M

AU - Pitsiladis, Y P

AU - Williams, A G

PY - 2017/1/19

Y1 - 2017/1/19

N2 - Background FTO gene variants have been associated with obesity phenotypes in sedentary and obese populations, but rarely with skeletal muscle and elite athlete phenotypes. Methods In 1089 participants, comprising 530 elite rugby athletes and 559 non-athletes, DNA was collected and genotyped for the FTO rs9939609 variant using real-time PCR. In a subgroup of non-resistance trained individuals (NT; n = 120), we also assessed structural and functional skeletal muscle phenotypes using dual energy x-ray absorptiometry, ultrasound and isokinetic dynamometry. In a subgroup of rugby athletes (n = 77), we assessed muscle power during a countermovement jump. Results In NT, TT genotype and T allele carriers had greater total body (4.8% and 4.1%) and total appendicular lean mass (LM; 3.0% and 2.1%) compared to AA genotype, with greater arm LM (0.8%) in T allele carriers and leg LM (2.1%) for TT, compared to AA genotype. Furthermore, the T allele was more common (94%) in selected elite rugby union athletes (back three and centre players) who are most reliant on LM rather than total body mass for success, compared to other rugby athletes (82%; P = 0.01, OR = 3.34) and controls (84%; P = 0.03, OR = 2.88). Accordingly, these athletes had greater peak power relative to body mass than other rugby athletes (14%; P = 2 x 10-6). Conclusion Collectively, these results suggest that the T allele is associated with increased LM and elite athletic success. This has implications for athletic populations, as well as conditions characterised by low LM such as sarcopenia and cachexia.

AB - Background FTO gene variants have been associated with obesity phenotypes in sedentary and obese populations, but rarely with skeletal muscle and elite athlete phenotypes. Methods In 1089 participants, comprising 530 elite rugby athletes and 559 non-athletes, DNA was collected and genotyped for the FTO rs9939609 variant using real-time PCR. In a subgroup of non-resistance trained individuals (NT; n = 120), we also assessed structural and functional skeletal muscle phenotypes using dual energy x-ray absorptiometry, ultrasound and isokinetic dynamometry. In a subgroup of rugby athletes (n = 77), we assessed muscle power during a countermovement jump. Results In NT, TT genotype and T allele carriers had greater total body (4.8% and 4.1%) and total appendicular lean mass (LM; 3.0% and 2.1%) compared to AA genotype, with greater arm LM (0.8%) in T allele carriers and leg LM (2.1%) for TT, compared to AA genotype. Furthermore, the T allele was more common (94%) in selected elite rugby union athletes (back three and centre players) who are most reliant on LM rather than total body mass for success, compared to other rugby athletes (82%; P = 0.01, OR = 3.34) and controls (84%; P = 0.03, OR = 2.88). Accordingly, these athletes had greater peak power relative to body mass than other rugby athletes (14%; P = 2 x 10-6). Conclusion Collectively, these results suggest that the T allele is associated with increased LM and elite athletic success. This has implications for athletic populations, as well as conditions characterised by low LM such as sarcopenia and cachexia.

KW - IRX3

KW - Lean mass

KW - RugbyGene project

U2 - 10.1186/s12863-017-0470-1

DO - 10.1186/s12863-017-0470-1

M3 - Article

VL - 18

JO - BMC Genetics

JF - BMC Genetics

SN - 1471-2156

IS - 4

ER -