Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment

Seyed Ali Poursamar, Javad Hatami, Alexander N Lehner, Claudio da Silva, Frederico Ferreira, A Paula M Antunes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The current study presents an effective and simple strategy to obtain stable porous scaffolds from gelatin via a gas foaming method. The technique exploits the intrinsic foaming ability of gelatin in the presence of CO2 to obtain a porous structure stabilised with glutaraldehyde. The produced scaffolds were characterised using physical and mechanical characterisation methods. The results showed that gas foaming may allow the tailoring of the 3-dimensional structure of the scaffolds with an interconnected porous structure. To assess the effectiveness of the preparation method in mitigating the potential cytotoxicity risk of using glutaraldehyde as a crosslinker, direct and in-direct cytotoxicity assays were performed at different concentrations of glutaraldehyde. The results indicate the potential of the gas foaming method, in the preparation of viable tissue engineering scaffolds.
Original languageEnglish
JournalMaterials Science and Engineering: C
Volume48
DOIs
Publication statusPublished - 1 Mar 2015

Fingerprint

Gas foaming
Glutaral
Gelatin
Scaffolds (biology)
Cytotoxicity
Scaffolds
Tissue Scaffolds
Tissue engineering
Assays

Keywords

  • crosslinking
  • denaturation temperature
  • gas foaming
  • gelatin
  • tensile strength

Cite this

Poursamar, Seyed Ali ; Hatami, Javad ; Lehner, Alexander N ; da Silva, Claudio ; Ferreira, Frederico ; Antunes, A Paula M. / Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment. In: Materials Science and Engineering: C. 2015 ; Vol. 48.
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Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment. / Poursamar, Seyed Ali; Hatami, Javad; Lehner, Alexander N; da Silva, Claudio; Ferreira, Frederico; Antunes, A Paula M.

In: Materials Science and Engineering: C, Vol. 48, 01.03.2015.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment

AU - Poursamar, Seyed Ali

AU - Hatami, Javad

AU - Lehner, Alexander N

AU - da Silva, Claudio

AU - Ferreira, Frederico

AU - Antunes, A Paula M

PY - 2015/3/1

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AB - The current study presents an effective and simple strategy to obtain stable porous scaffolds from gelatin via a gas foaming method. The technique exploits the intrinsic foaming ability of gelatin in the presence of CO2 to obtain a porous structure stabilised with glutaraldehyde. The produced scaffolds were characterised using physical and mechanical characterisation methods. The results showed that gas foaming may allow the tailoring of the 3-dimensional structure of the scaffolds with an interconnected porous structure. To assess the effectiveness of the preparation method in mitigating the potential cytotoxicity risk of using glutaraldehyde as a crosslinker, direct and in-direct cytotoxicity assays were performed at different concentrations of glutaraldehyde. The results indicate the potential of the gas foaming method, in the preparation of viable tissue engineering scaffolds.

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