High fat diet induced obesity modulates melanoma tumour microenvironment in low density lipoprotein receptor deficient LDLR-/- mouse model.

Rafah Al-Zubaidi, Cordula Stover, Lee Richard Machado

    Research output: Contribution to conference typesAbstractResearch

    Abstract

    Inflammation and altered immune response are the main features of obesity and contribute greatly to the promotion of obesityrelated metabolic complications, especially cancer development and progression. Adipose tissue expansion is associated with increased tumour infiltration by regulatory T cells (T-regs) which are critical regulators of the adaptive immune response. Adipocytes and infiltrating immune cells secrete pro-inflammatory adipokines and cytokines providing a microenvironment favourable for tumour growth. LDLR-/- mice fed on high fat diet and control diet were subcutaneously injected with 5??105 syngeneic melanoma cells (B16F10). After two weeks, tumours and spleens were dissected. Tumours and bodies were weighed at endpoint and then the percentage of CD4+CD25+FOXP3+T-regs population among splenocytes was determined by flow cytometry (FACS). High Fat Diet (HFD) feeding increases solid tumour growth combined with increases in adipose tissues of LDLR-/- tumour bearing mice. The percentage of T-regs among spleen lymphocytes was significantly higher in tumour bearing mice fed on high fat diet compared with those fed on control diet. Obesity may promote tumour progression by favouring an immune suppressive tumour microenvironment.
    Original languageEnglish
    Pages90
    Number of pages1
    DOIs
    Publication statusPublished - 13 Sep 2018
    Event4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry
    - London, United Kingdom
    Duration: 13 Sep 201815 Sep 2018

    Conference

    Conference4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry
    CountryUnited Kingdom
    CityLondon
    Period13/09/1815/09/18

    Fingerprint

    Tumor Microenvironment
    LDL Receptors
    High Fat Diet
    Melanoma
    Obesity
    Neoplasms
    Adipose Tissue
    Spleen
    Tissue Expansion
    Diet
    Adipokines
    Adaptive Immunity
    Regulatory T-Lymphocytes
    Growth
    Adipocytes
    Flow Cytometry
    Lymphocytes
    Cytokines
    Inflammation

    Cite this

    Al-Zubaidi, R., Stover, C., & Machado, L. R. (2018). High fat diet induced obesity modulates melanoma tumour microenvironment in low density lipoprotein receptor deficient LDLR-/- mouse model.. 90. Abstract from 4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry, London, United Kingdom. https://doi.org/10.4172/2155-9600-C7-072
    Al-Zubaidi, Rafah ; Stover, Cordula ; Machado, Lee Richard. / High fat diet induced obesity modulates melanoma tumour microenvironment in low density lipoprotein receptor deficient LDLR-/- mouse model. Abstract from 4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry, London, United Kingdom.1 p.
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    abstract = "Inflammation and altered immune response are the main features of obesity and contribute greatly to the promotion of obesityrelated metabolic complications, especially cancer development and progression. Adipose tissue expansion is associated with increased tumour infiltration by regulatory T cells (T-regs) which are critical regulators of the adaptive immune response. Adipocytes and infiltrating immune cells secrete pro-inflammatory adipokines and cytokines providing a microenvironment favourable for tumour growth. LDLR-/- mice fed on high fat diet and control diet were subcutaneously injected with 5??105 syngeneic melanoma cells (B16F10). After two weeks, tumours and spleens were dissected. Tumours and bodies were weighed at endpoint and then the percentage of CD4+CD25+FOXP3+T-regs population among splenocytes was determined by flow cytometry (FACS). High Fat Diet (HFD) feeding increases solid tumour growth combined with increases in adipose tissues of LDLR-/- tumour bearing mice. The percentage of T-regs among spleen lymphocytes was significantly higher in tumour bearing mice fed on high fat diet compared with those fed on control diet. Obesity may promote tumour progression by favouring an immune suppressive tumour microenvironment.",
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    Al-Zubaidi, R, Stover, C & Machado, LR 2018, 'High fat diet induced obesity modulates melanoma tumour microenvironment in low density lipoprotein receptor deficient LDLR-/- mouse model.' 4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry, London, United Kingdom, 13/09/18 - 15/09/18, pp. 90. https://doi.org/10.4172/2155-9600-C7-072

    High fat diet induced obesity modulates melanoma tumour microenvironment in low density lipoprotein receptor deficient LDLR-/- mouse model. / Al-Zubaidi, Rafah; Stover, Cordula; Machado, Lee Richard.

    2018. 90 Abstract from 4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry, London, United Kingdom.

    Research output: Contribution to conference typesAbstractResearch

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    AU - Stover, Cordula

    AU - Machado, Lee Richard

    PY - 2018/9/13

    Y1 - 2018/9/13

    N2 - Inflammation and altered immune response are the main features of obesity and contribute greatly to the promotion of obesityrelated metabolic complications, especially cancer development and progression. Adipose tissue expansion is associated with increased tumour infiltration by regulatory T cells (T-regs) which are critical regulators of the adaptive immune response. Adipocytes and infiltrating immune cells secrete pro-inflammatory adipokines and cytokines providing a microenvironment favourable for tumour growth. LDLR-/- mice fed on high fat diet and control diet were subcutaneously injected with 5??105 syngeneic melanoma cells (B16F10). After two weeks, tumours and spleens were dissected. Tumours and bodies were weighed at endpoint and then the percentage of CD4+CD25+FOXP3+T-regs population among splenocytes was determined by flow cytometry (FACS). High Fat Diet (HFD) feeding increases solid tumour growth combined with increases in adipose tissues of LDLR-/- tumour bearing mice. The percentage of T-regs among spleen lymphocytes was significantly higher in tumour bearing mice fed on high fat diet compared with those fed on control diet. Obesity may promote tumour progression by favouring an immune suppressive tumour microenvironment.

    AB - Inflammation and altered immune response are the main features of obesity and contribute greatly to the promotion of obesityrelated metabolic complications, especially cancer development and progression. Adipose tissue expansion is associated with increased tumour infiltration by regulatory T cells (T-regs) which are critical regulators of the adaptive immune response. Adipocytes and infiltrating immune cells secrete pro-inflammatory adipokines and cytokines providing a microenvironment favourable for tumour growth. LDLR-/- mice fed on high fat diet and control diet were subcutaneously injected with 5??105 syngeneic melanoma cells (B16F10). After two weeks, tumours and spleens were dissected. Tumours and bodies were weighed at endpoint and then the percentage of CD4+CD25+FOXP3+T-regs population among splenocytes was determined by flow cytometry (FACS). High Fat Diet (HFD) feeding increases solid tumour growth combined with increases in adipose tissues of LDLR-/- tumour bearing mice. The percentage of T-regs among spleen lymphocytes was significantly higher in tumour bearing mice fed on high fat diet compared with those fed on control diet. Obesity may promote tumour progression by favouring an immune suppressive tumour microenvironment.

    UR - https://www.omicsonline.org/conference-proceedings/nutri-food-chemistry-euro-obesity-2018-posters.digital

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    Al-Zubaidi R, Stover C, Machado LR. High fat diet induced obesity modulates melanoma tumour microenvironment in low density lipoprotein receptor deficient LDLR-/- mouse model.. 2018. Abstract from 4th Euro Obesity and Endocrinology Congress
    17th World Congress on Nutrition and Food Chemistry, London, United Kingdom. https://doi.org/10.4172/2155-9600-C7-072