Infusing advanced cancer patients with tumour antigen-specific CD4+ and CD8+ T cell clones

S P Lee, A M Green, Lee Machado, P Patel, Wendy Thomas, O Goodyear, D Briggs, D McDonald, J Marsden, Neil Steven

Research output: Contribution to ConferenceAbstract

Abstract

Previous attempts to treat human cancer by infusing tumour antigen- specific T cells have highlighted the need to deliver CD4+ T cells to maintain an effective CD8+ T cell response in vivo. We have explored a protocol designed to generate both CD4+ and CD8+ T cell clones for infusing advanced cancer patients. T cells were reactivated in vitro from patients with EBV-associated tumours, melanoma and prostate cancer using DCs pulsed with peptides representing defined epitopes within tumour antigens. Cloning efficiency was significantly improved by pre-selecting specific cells using an interferon capture assay. CD4+ and/or CD8+ T cell clones were successfully generated from 16/27 patients, six of whom were infused with escalating doses of T cells. Treatments were well tolerated, and functional cells were detectable in blood 1-14 days postinfusion, with some cells present for >2 months. T cell clones were heterogeneous both in their ability to recognise tumour lines and in their homing phenotype. This highlights the value of cloning T cell populations which can be screened prior to infusion to select those with sufficient avidity for the target antigen and with the appropriate homing phenotype.
Original languageEnglish
Publication statusPublished - 1 Jan 2005
EventAnnual Congress of the British Society for Immunology - Liverpool, UK
Duration: 1 Dec 2011 → …

Conference

ConferenceAnnual Congress of the British Society for Immunology
Period1/12/11 → …

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