Mitochondrial respiration is decreased in rat kidney following fetal exposure to a maternal low-protein diet

S Engeham, Kennedy Mdaki, K Jewell, R Austin, Alexander N Lehner, Simon C Langley-Evans

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Maternal protein restriction in rat pregnancy is associated with impaired renal development and age-related loss of renal function in the resulting offspring. Pregnant rats were fed either control or low-protein (LP) diets, and kidneys from their male offspring were collected at 4, 13, or 16 weeks of age. Mitochondrial state 3 and state 4 respiratory rates were decreased by a third in the LP exposed adults. The reduction in mitochondrial function was not explained by complex IV deficiency or altered expression of the complex I subunits that are typically associated with mitochondrial dysfunction. Similarly, there was no evidence that LP-exposure resulted in greater oxidative damage to the kidney, differential expression of ATP synthetase β-subunit, and ATP-ADP translocase 1. mRNA expression of uncoupling protein 2 was increased in adult rats exposed to LP in utero, but there was no evidence of differential expression at the protein level. Exposure to maternal undernutrition is associated with a decrease in mitochondrial respiration in kidneys of adult rats. In the absence of gross disturbances in respiratory chain protein expression, programming of coupling efficiency may explain the long-term impact of the maternal diet.
Original languageEnglish
JournalJournal of Nutrition and Metabolism
Volume2012
DOIs
Publication statusPublished - 27 Mar 2012

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Protein-Restricted Diet
Respiration
Mothers
Kidney
Proteins
Cytochrome-c Oxidase Deficiency
ATP Translocases Mitochondrial ADP
Maternal Exposure
Ligases
Respiratory Rate
Electron Transport
Malnutrition
Adenosine Triphosphate
Diet
Pregnancy
Messenger RNA

Cite this

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title = "Mitochondrial respiration is decreased in rat kidney following fetal exposure to a maternal low-protein diet",
abstract = "Maternal protein restriction in rat pregnancy is associated with impaired renal development and age-related loss of renal function in the resulting offspring. Pregnant rats were fed either control or low-protein (LP) diets, and kidneys from their male offspring were collected at 4, 13, or 16 weeks of age. Mitochondrial state 3 and state 4 respiratory rates were decreased by a third in the LP exposed adults. The reduction in mitochondrial function was not explained by complex IV deficiency or altered expression of the complex I subunits that are typically associated with mitochondrial dysfunction. Similarly, there was no evidence that LP-exposure resulted in greater oxidative damage to the kidney, differential expression of ATP synthetase β-subunit, and ATP-ADP translocase 1. mRNA expression of uncoupling protein 2 was increased in adult rats exposed to LP in utero, but there was no evidence of differential expression at the protein level. Exposure to maternal undernutrition is associated with a decrease in mitochondrial respiration in kidneys of adult rats. In the absence of gross disturbances in respiratory chain protein expression, programming of coupling efficiency may explain the long-term impact of the maternal diet.",
author = "S Engeham and Kennedy Mdaki and K Jewell and R Austin and Lehner, {Alexander N} and Langley-Evans, {Simon C}",
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doi = "10.1155/2012/989037",
language = "English",
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journal = "Journal of Nutrition and Metabolism",
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Mitochondrial respiration is decreased in rat kidney following fetal exposure to a maternal low-protein diet. / Engeham, S; Mdaki, Kennedy; Jewell, K; Austin, R; Lehner, Alexander N; Langley-Evans, Simon C.

In: Journal of Nutrition and Metabolism, Vol. 2012, 27.03.2012.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Mdaki, Kennedy

AU - Jewell, K

AU - Austin, R

AU - Lehner, Alexander N

AU - Langley-Evans, Simon C

PY - 2012/3/27

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N2 - Maternal protein restriction in rat pregnancy is associated with impaired renal development and age-related loss of renal function in the resulting offspring. Pregnant rats were fed either control or low-protein (LP) diets, and kidneys from their male offspring were collected at 4, 13, or 16 weeks of age. Mitochondrial state 3 and state 4 respiratory rates were decreased by a third in the LP exposed adults. The reduction in mitochondrial function was not explained by complex IV deficiency or altered expression of the complex I subunits that are typically associated with mitochondrial dysfunction. Similarly, there was no evidence that LP-exposure resulted in greater oxidative damage to the kidney, differential expression of ATP synthetase β-subunit, and ATP-ADP translocase 1. mRNA expression of uncoupling protein 2 was increased in adult rats exposed to LP in utero, but there was no evidence of differential expression at the protein level. Exposure to maternal undernutrition is associated with a decrease in mitochondrial respiration in kidneys of adult rats. In the absence of gross disturbances in respiratory chain protein expression, programming of coupling efficiency may explain the long-term impact of the maternal diet.

AB - Maternal protein restriction in rat pregnancy is associated with impaired renal development and age-related loss of renal function in the resulting offspring. Pregnant rats were fed either control or low-protein (LP) diets, and kidneys from their male offspring were collected at 4, 13, or 16 weeks of age. Mitochondrial state 3 and state 4 respiratory rates were decreased by a third in the LP exposed adults. The reduction in mitochondrial function was not explained by complex IV deficiency or altered expression of the complex I subunits that are typically associated with mitochondrial dysfunction. Similarly, there was no evidence that LP-exposure resulted in greater oxidative damage to the kidney, differential expression of ATP synthetase β-subunit, and ATP-ADP translocase 1. mRNA expression of uncoupling protein 2 was increased in adult rats exposed to LP in utero, but there was no evidence of differential expression at the protein level. Exposure to maternal undernutrition is associated with a decrease in mitochondrial respiration in kidneys of adult rats. In the absence of gross disturbances in respiratory chain protein expression, programming of coupling efficiency may explain the long-term impact of the maternal diet.

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