Overexpression of the dopamine receptor‐interacting protein Alix/ AIP 1 modulates NMDA receptor‐triggered cell death

Research output: Contribution to JournalArticle

Abstract

Alix/AIP1 is an adaptor protein involved in apoptosis, endocytic membrane trafficking and brain development. Alix has been found within the human postsynaptic density (PSD) and, since NMDA receptors (NMDARs) are central components of the PSD, we hypothesized that the close proximity of both proteins may allow Alix to influence the downstream pathways following NMDAR activation. NMDARs play important roles in excitotoxicity and we evaluated the effects of recombinant Alix in an NMDAR cell death assay. Overexpression of Alix with NMDARs increases the potency of NMDAR‐ induced cell death compared to cells expressing only NMDARs, and this requires expression of the Alix C‐terminal region. Therefore, we demonstrate a previously unreported role for Alix as a potential modulator of NMDAR function.
Original languageEnglish
Pages (from-to)1381-1391
JournalFEBS Letters
Volume593
Issue number12
Early online date24 May 2019
DOIs
Publication statusPublished - 1 Jun 2019

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Cell death
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Dopamine
Cell Death
Proteins
Post-Synaptic Density
Modulators
Assays
Brain
Chemical activation
Cells
Apoptosis
Membranes

Keywords

  • dopamine

Cite this

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abstract = "Alix/AIP1 is an adaptor protein involved in apoptosis, endocytic membrane trafficking and brain development. Alix has been found within the human postsynaptic density (PSD) and, since NMDA receptors (NMDARs) are central components of the PSD, we hypothesized that the close proximity of both proteins may allow Alix to influence the downstream pathways following NMDAR activation. NMDARs play important roles in excitotoxicity and we evaluated the effects of recombinant Alix in an NMDAR cell death assay. Overexpression of Alix with NMDARs increases the potency of NMDAR‐ induced cell death compared to cells expressing only NMDARs, and this requires expression of the Alix C‐terminal region. Therefore, we demonstrate a previously unreported role for Alix as a potential modulator of NMDAR function.",
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Overexpression of the dopamine receptor‐interacting protein Alix/ AIP 1 modulates NMDA receptor‐triggered cell death. / Nasir, Jamal.

In: FEBS Letters, Vol. 593, No. 12, 01.06.2019, p. 1381-1391.

Research output: Contribution to JournalArticle

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