Overexpression of the dopamine receptor‐interacting protein Alix/ AIP 1 modulates NMDA receptor‐triggered cell death

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Alix/AIP1 is an adaptor protein involved in apoptosis, endocytic membrane trafficking and brain development. Alix has been found within the human postsynaptic density (PSD) and, since NMDA receptors (NMDARs) are central components of the PSD, we hypothesized that the close proximity of both proteins may allow Alix to influence the downstream pathways following NMDAR activation. NMDARs play important roles in excitotoxicity and we evaluated the effects of recombinant Alix in an NMDAR cell death assay. Overexpression of Alix with NMDARs increases the potency of NMDAR‐ induced cell death compared to cells expressing only NMDARs, and this requires expression of the Alix C‐terminal region. Therefore, we demonstrate a previously unreported role for Alix as a potential modulator of NMDAR function.
    Original languageEnglish
    Pages (from-to)1381-1391
    JournalFEBS Letters
    Volume593
    Issue number12
    Early online date24 May 2019
    DOIs
    Publication statusPublished - 1 Jun 2019

    Fingerprint

    Cell death
    N-Methylaspartate
    N-Methyl-D-Aspartate Receptors
    Dopamine
    Cell Death
    Proteins
    Post-Synaptic Density
    Modulators
    Assays
    Brain
    Chemical activation
    Cells
    Apoptosis
    Membranes

    Keywords

    • dopamine

    Cite this

    @article{3882dd3acd8d4731853c6adf354b2b46,
    title = "Overexpression of the dopamine receptor‐interacting protein Alix/ AIP 1 modulates NMDA receptor‐triggered cell death",
    abstract = "Alix/AIP1 is an adaptor protein involved in apoptosis, endocytic membrane trafficking and brain development. Alix has been found within the human postsynaptic density (PSD) and, since NMDA receptors (NMDARs) are central components of the PSD, we hypothesized that the close proximity of both proteins may allow Alix to influence the downstream pathways following NMDAR activation. NMDARs play important roles in excitotoxicity and we evaluated the effects of recombinant Alix in an NMDAR cell death assay. Overexpression of Alix with NMDARs increases the potency of NMDAR‐ induced cell death compared to cells expressing only NMDARs, and this requires expression of the Alix C‐terminal region. Therefore, we demonstrate a previously unreported role for Alix as a potential modulator of NMDAR function.",
    keywords = "dopamine",
    author = "Jamal Nasir",
    year = "2019",
    month = "6",
    day = "1",
    doi = "10.1002/1873-3468.13434",
    language = "English",
    volume = "593",
    pages = "1381--1391",
    journal = "FEBS Letters",
    issn = "0014-5793",
    publisher = "Wiley",
    number = "12",

    }

    Overexpression of the dopamine receptor‐interacting protein Alix/ AIP 1 modulates NMDA receptor‐triggered cell death. / Nasir, Jamal.

    In: FEBS Letters, Vol. 593, No. 12, 01.06.2019, p. 1381-1391.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Overexpression of the dopamine receptor‐interacting protein Alix/ AIP 1 modulates NMDA receptor‐triggered cell death

    AU - Nasir, Jamal

    PY - 2019/6/1

    Y1 - 2019/6/1

    N2 - Alix/AIP1 is an adaptor protein involved in apoptosis, endocytic membrane trafficking and brain development. Alix has been found within the human postsynaptic density (PSD) and, since NMDA receptors (NMDARs) are central components of the PSD, we hypothesized that the close proximity of both proteins may allow Alix to influence the downstream pathways following NMDAR activation. NMDARs play important roles in excitotoxicity and we evaluated the effects of recombinant Alix in an NMDAR cell death assay. Overexpression of Alix with NMDARs increases the potency of NMDAR‐ induced cell death compared to cells expressing only NMDARs, and this requires expression of the Alix C‐terminal region. Therefore, we demonstrate a previously unreported role for Alix as a potential modulator of NMDAR function.

    AB - Alix/AIP1 is an adaptor protein involved in apoptosis, endocytic membrane trafficking and brain development. Alix has been found within the human postsynaptic density (PSD) and, since NMDA receptors (NMDARs) are central components of the PSD, we hypothesized that the close proximity of both proteins may allow Alix to influence the downstream pathways following NMDAR activation. NMDARs play important roles in excitotoxicity and we evaluated the effects of recombinant Alix in an NMDAR cell death assay. Overexpression of Alix with NMDARs increases the potency of NMDAR‐ induced cell death compared to cells expressing only NMDARs, and this requires expression of the Alix C‐terminal region. Therefore, we demonstrate a previously unreported role for Alix as a potential modulator of NMDAR function.

    KW - dopamine

    U2 - 10.1002/1873-3468.13434

    DO - 10.1002/1873-3468.13434

    M3 - Article

    VL - 593

    SP - 1381

    EP - 1391

    JO - FEBS Letters

    JF - FEBS Letters

    SN - 0014-5793

    IS - 12

    ER -