Structural analysis of the 5′ region of mouse and human huntington disease genes reveals conservation of putative promoter region and di- and trinucleotide polymorphisms

Biaoyang Lin, Jamal Nasir, Michael A. Kalchman, Helen McDonald, Jutta Zeisler, Y. Paul Goldberg, Michael R. Hayden

    Research output: Contribution to journalArticle

    Abstract

    We have previously cloned and characterized the murine homologue of the Huntington disease (HD) gene and shown that it maps to mouse chromosome 5 within a region of conserved synteny with human chromosome 4p16.3. Here we present a detailed comparison of the sequence of the putative promoter and the organization of the 5′ genomic region of the murine (Hdh) and human HD genes encompassing the first five exons. We show that in this region these two genes share identical exon boundaries, but have differentsize introns. Two dinucleotide (CT) and one trinucleotide intronic polymorphism in Hdh and an intronic CA polymorphism in the HD gene were identified. Comparison of 940-bp sequence 5′ to the putative translation start site reveals a highly conserved region (78.8% nucleotide identity) between Hdh and the HD gene from nucleotide -56 to -206 (of Hdh). Neither Hdh nor the HD gene have typical TATA or CCAAT elements, but both show one putative AP2 binding site and numerous potential Sp1 binding sites. The high sequence identity between Hdh and the HD gene for approximately 200 bp 5′ to the putative translation start site indicates that these sequences may play a role in regulating expression of the Huntington disease gene. © 1995 Academic Press, Inc.
    Original languageEnglish
    Pages (from-to)707-715
    Number of pages9
    JournalBMC Genomics
    Volume25
    Issue number3
    DOIs
    Publication statusPublished - 1995

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    Huntington Disease
    Genetic Promoter Regions
    Genes
    Exons
    Nucleotides
    Binding Sites
    Synteny
    Chromosomes, Human, Pair 5
    Human Chromosomes
    Introns

    Cite this

    Lin, Biaoyang ; Nasir, Jamal ; Kalchman, Michael A. ; McDonald, Helen ; Zeisler, Jutta ; Goldberg, Y. Paul ; Hayden, Michael R. / Structural analysis of the 5′ region of mouse and human huntington disease genes reveals conservation of putative promoter region and di- and trinucleotide polymorphisms. In: BMC Genomics. 1995 ; Vol. 25, No. 3. pp. 707-715.
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    abstract = "We have previously cloned and characterized the murine homologue of the Huntington disease (HD) gene and shown that it maps to mouse chromosome 5 within a region of conserved synteny with human chromosome 4p16.3. Here we present a detailed comparison of the sequence of the putative promoter and the organization of the 5′ genomic region of the murine (Hdh) and human HD genes encompassing the first five exons. We show that in this region these two genes share identical exon boundaries, but have differentsize introns. Two dinucleotide (CT) and one trinucleotide intronic polymorphism in Hdh and an intronic CA polymorphism in the HD gene were identified. Comparison of 940-bp sequence 5′ to the putative translation start site reveals a highly conserved region (78.8{\%} nucleotide identity) between Hdh and the HD gene from nucleotide -56 to -206 (of Hdh). Neither Hdh nor the HD gene have typical TATA or CCAAT elements, but both show one putative AP2 binding site and numerous potential Sp1 binding sites. The high sequence identity between Hdh and the HD gene for approximately 200 bp 5′ to the putative translation start site indicates that these sequences may play a role in regulating expression of the Huntington disease gene. {\circledC} 1995 Academic Press, Inc.",
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    Structural analysis of the 5′ region of mouse and human huntington disease genes reveals conservation of putative promoter region and di- and trinucleotide polymorphisms. / Lin, Biaoyang; Nasir, Jamal; Kalchman, Michael A.; McDonald, Helen; Zeisler, Jutta; Goldberg, Y. Paul; Hayden, Michael R.

    In: BMC Genomics, Vol. 25, No. 3, 1995, p. 707-715.

    Research output: Contribution to journalArticle

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