The degree of acceptability of swine blood values at increasing levels of hemolysis evaluated through visual inspection versus automated quantification.

Martino G Di, AL Stefani, G Lippi, L Gagliazzo, W McCormick, G Gabai, L Bonfanti

Research output: Contribution to JournalArticle

Abstract

The pronounced fragility that characterizes swine erythrocytes is likely to produce a variable degree of hemolysis during blood sampling, and the free hemoglobin may then unpredictably bias the quantification of several analytes. The aim of this study was to evaluate the degree of acceptability of values obtained for several biochemical parameters at different levels of hemolysis. Progressively increased degrees of physical hemolysis were induced in 3 aliquots of 30 nonhemolytic sera, and the relative effects on the test results were assessed. To define the level of hemolysis, we used both visual estimation (on a scale of 0 to 3+) and analytical assessment (hemolytic index) and identified the best analytical cutoff values for discriminating the visual levels of hemolysis. Hemolysis led to a variable and dose-dependent effect on the test results that was specific for each analyte tested. In mildly hemolyzed specimens, C-reactive protein, haptoglobin, β1-globulin, β2-globulin, α1-globulin, γ-globulin, sodium, calcium, and alkaline phosphatase were not significantly biased, whereas α2-globulin, albumin, urea, creatinine, glucose, total cholesterol, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, nonesterified fatty acids, bilirubin, phosphorus, magnesium, iron, zinc, copper, lipase, triglycerides, lactate dehydrogenase, unbound iron-binding capacity, and uric acid were significantly biased. Chloride and total protein were unbiased even in markedly hemolyzed samples. Analytical interference was hypothesized to be the main source of this bias, leading to a nonlinear trend that confirmed the difficulty in establishing reliable coefficients of correction for adjusting the test results.
Original languageEnglish
Pages (from-to)306
Number of pages312
JournalJournal of Veterinary Diagnostic Investigation
Volume27
Issue number3
DOIs
Publication statusPublished - 2 Jun 2015

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Hemolysis
Globulins
Swine
Iron
Haptoglobins
Transferases
Aspartate Aminotransferases
Uric Acid
Lipase
Alanine Transaminase
L-Lactate Dehydrogenase
Bilirubin
Nonesterified Fatty Acids
C-Reactive Protein
Phosphorus
Magnesium
Alkaline Phosphatase
Urea
Zinc
Chlorides

Keywords

  • biochemical profile
  • C-reactive protein
  • haptoglobin
  • hemolysis
  • swine

Cite this

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title = "The degree of acceptability of swine blood values at increasing levels of hemolysis evaluated through visual inspection versus automated quantification.",
abstract = "The pronounced fragility that characterizes swine erythrocytes is likely to produce a variable degree of hemolysis during blood sampling, and the free hemoglobin may then unpredictably bias the quantification of several analytes. The aim of this study was to evaluate the degree of acceptability of values obtained for several biochemical parameters at different levels of hemolysis. Progressively increased degrees of physical hemolysis were induced in 3 aliquots of 30 nonhemolytic sera, and the relative effects on the test results were assessed. To define the level of hemolysis, we used both visual estimation (on a scale of 0 to 3+) and analytical assessment (hemolytic index) and identified the best analytical cutoff values for discriminating the visual levels of hemolysis. Hemolysis led to a variable and dose-dependent effect on the test results that was specific for each analyte tested. In mildly hemolyzed specimens, C-reactive protein, haptoglobin, β1-globulin, β2-globulin, α1-globulin, γ-globulin, sodium, calcium, and alkaline phosphatase were not significantly biased, whereas α2-globulin, albumin, urea, creatinine, glucose, total cholesterol, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, nonesterified fatty acids, bilirubin, phosphorus, magnesium, iron, zinc, copper, lipase, triglycerides, lactate dehydrogenase, unbound iron-binding capacity, and uric acid were significantly biased. Chloride and total protein were unbiased even in markedly hemolyzed samples. Analytical interference was hypothesized to be the main source of this bias, leading to a nonlinear trend that confirmed the difficulty in establishing reliable coefficients of correction for adjusting the test results.",
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The degree of acceptability of swine blood values at increasing levels of hemolysis evaluated through visual inspection versus automated quantification. / Di, Martino G; Stefani, AL; Lippi, G; Gagliazzo, L; McCormick, W; Gabai, G; Bonfanti, L.

In: Journal of Veterinary Diagnostic Investigation, Vol. 27, No. 3, 02.06.2015, p. 306.

Research output: Contribution to JournalArticle

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T1 - The degree of acceptability of swine blood values at increasing levels of hemolysis evaluated through visual inspection versus automated quantification.

AU - Di, Martino G

AU - Stefani, AL

AU - Lippi, G

AU - Gagliazzo, L

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AU - Gabai, G

AU - Bonfanti, L

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