Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow

Umaimainthan Palendira, Rosanna Chinn, Wajid Raza, Karen Piper, Guy Pratt, Lee Machado, Andrew Bell, Naeem Khan, Andrew Hislop, Richard Steyn, Alan Rickinson, Christopher Buckley, Paul Moss

Research output: Contribution to journalArticleResearch

Abstract

The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8(+) T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8(+) T cells showed widely divergent partition of antigen-specific population between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalovirus (CMV)–specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60% within marrow. Bone marrow T cells were found to exhibit a unique CCR5(+)CXCR6(+)CXCR3(-) homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. Expression of CCR5 and CXCR6 was higher on EBV-specific T cells within peripheral blood compared with CMV-specific populations. These observations identify a novel bone marrow homing phenotype for CD8(+) memory T cells, which necessitates a reevaluation of the magnitude of antigen-specific populations within the lymphoid system.
Original languageEnglish
JournalBlood
Volume112
Issue number8
DOIs
Publication statusPublished - 15 Oct 2008

Fingerprint

Bone Marrow
Viruses
T-Lymphocytes
Phenotype
Human Herpesvirus 4
Cytomegalovirus
Antigens
Population
Viral Load
Bone Marrow Cells
Epitopes
Immune System
Proteins

Cite this

Palendira, Umaimainthan ; Chinn, Rosanna ; Raza, Wajid ; Piper, Karen ; Pratt, Guy ; Machado, Lee ; Bell, Andrew ; Khan, Naeem ; Hislop, Andrew ; Steyn, Richard ; Rickinson, Alan ; Buckley, Christopher ; Moss, Paul. / Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow. In: Blood. 2008 ; Vol. 112, No. 8.
@article{fc561077a62b49fba92ee53946df840f,
title = "Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow",
abstract = "The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8(+) T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8(+) T cells showed widely divergent partition of antigen-specific population between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalovirus (CMV)–specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60{\%} within marrow. Bone marrow T cells were found to exhibit a unique CCR5(+)CXCR6(+)CXCR3(-) homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. Expression of CCR5 and CXCR6 was higher on EBV-specific T cells within peripheral blood compared with CMV-specific populations. These observations identify a novel bone marrow homing phenotype for CD8(+) memory T cells, which necessitates a reevaluation of the magnitude of antigen-specific populations within the lymphoid system.",
author = "Umaimainthan Palendira and Rosanna Chinn and Wajid Raza and Karen Piper and Guy Pratt and Lee Machado and Andrew Bell and Naeem Khan and Andrew Hislop and Richard Steyn and Alan Rickinson and Christopher Buckley and Paul Moss",
year = "2008",
month = "10",
day = "15",
doi = "10.1182/blood-2008-02-138040",
language = "English",
volume = "112",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "8",

}

Palendira, U, Chinn, R, Raza, W, Piper, K, Pratt, G, Machado, L, Bell, A, Khan, N, Hislop, A, Steyn, R, Rickinson, A, Buckley, C & Moss, P 2008, 'Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow', Blood, vol. 112, no. 8. https://doi.org/10.1182/blood-2008-02-138040

Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow. / Palendira, Umaimainthan; Chinn, Rosanna; Raza, Wajid; Piper, Karen; Pratt, Guy; Machado, Lee; Bell, Andrew; Khan, Naeem; Hislop, Andrew; Steyn, Richard; Rickinson, Alan; Buckley, Christopher; Moss, Paul.

In: Blood, Vol. 112, No. 8, 15.10.2008.

Research output: Contribution to journalArticleResearch

TY - JOUR

T1 - Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow

AU - Palendira, Umaimainthan

AU - Chinn, Rosanna

AU - Raza, Wajid

AU - Piper, Karen

AU - Pratt, Guy

AU - Machado, Lee

AU - Bell, Andrew

AU - Khan, Naeem

AU - Hislop, Andrew

AU - Steyn, Richard

AU - Rickinson, Alan

AU - Buckley, Christopher

AU - Moss, Paul

PY - 2008/10/15

Y1 - 2008/10/15

N2 - The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8(+) T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8(+) T cells showed widely divergent partition of antigen-specific population between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalovirus (CMV)–specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60% within marrow. Bone marrow T cells were found to exhibit a unique CCR5(+)CXCR6(+)CXCR3(-) homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. Expression of CCR5 and CXCR6 was higher on EBV-specific T cells within peripheral blood compared with CMV-specific populations. These observations identify a novel bone marrow homing phenotype for CD8(+) memory T cells, which necessitates a reevaluation of the magnitude of antigen-specific populations within the lymphoid system.

AB - The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8(+) T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8(+) T cells showed widely divergent partition of antigen-specific population between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalovirus (CMV)–specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60% within marrow. Bone marrow T cells were found to exhibit a unique CCR5(+)CXCR6(+)CXCR3(-) homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. Expression of CCR5 and CXCR6 was higher on EBV-specific T cells within peripheral blood compared with CMV-specific populations. These observations identify a novel bone marrow homing phenotype for CD8(+) memory T cells, which necessitates a reevaluation of the magnitude of antigen-specific populations within the lymphoid system.

U2 - 10.1182/blood-2008-02-138040

DO - 10.1182/blood-2008-02-138040

M3 - Article

VL - 112

JO - Blood

JF - Blood

SN - 0006-4971

IS - 8

ER -